A rather generic and outdated description of what we do in the lab.
A newer but incomplete description of what we do in the lab can be found here.

General Protocols

  • PCR protocol submitted by Igor Brodsky (9/12/00)
  • Tissue culture cell fixation submitted by Igor Brodsky (9/12/00)
  • Immunofluorescence assay submitted by Denise Monack (9/12/00)
  • Tissue preparation for microscopy submitted by Denise Monack (9/12/00)
  • Total DNA Isolation submitted by Charlie Kim (9/12/02)
  • Modified RNeasy Miniprep submitted by Charlie Kim (9/12/02)

    Salmonella Protocols

  • Defined medias submitted by Igor Brodsky (9/12/00)
  • Invasion/Replication assay submitted by Igor Brodsky (9/12/00)
  • Replication assay submitted by Igor Brodsky (4/7/04)
  • FACS of infected macrophages submitted by Igor Brodsky (9/12/00)
  • P22 Transduction submitted by Anthea Lee (9/12/00)
  • Knockouts a la Datsenko & Wanner submitted by Charlie Kim (6/14/02)
  • The Roth Lab Protocols Page (this page is excellent)

    Yersinia Protocols

  • Invasion assay submitted by Denise Monack (9/12/00)

    Computer Protocols

  • Get a CMGM account
  • Keyserver Software
  • E-mail forwarding and auto-replying
  • CDL Updates

    Primers for the Wanner Knockout Method

    Software from the Falkow Lab

    Supplementary Data



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